The sites are located in strong town and city centre locations within England, Scotland and Wales.
CBRE and Savills have been appointed to sell the 33 pubs, along with 12 others which the pubco put on the market last year.
Of all 45 appointed to the agents, 23 are in the south or London.
A spokesperson on behalf of the joint agents said: "The portfolio represents an excellent opportunity to acquire high-volume businesses with strong food sales in established locations, which are fitted to an extremely high standard.
"We therefore anticipate a broad range of interest from pub, bar and restaurant groups."
JDW, which owns close to 1,000 pubs, has sold sites to Amber Taverns, Brewhouse & Kitchen, Hawthorn Leisure, Stonegate and Urban Pubs & Bars in recent months.
The latest pubs for sale are:
- Banbury - Fleur-de-Lis
- Bathgate - James Young
- Berwick-upon-Tweed - Leaping Salmon
- Birmingham - Soloman Cutler
- Bootle - Merton Inn
- Boscombe - Sir Percy Florence Shelley
- Bradford - Sir Titus Salt
- Cheadle Hulme - Kings Hall
- Dagenham - Lord Denman
- Dartford - Paper Moon
- Didsbury - Milson Rhodes
- Ellesmere Port - Thomas Telford
- Forest Hill - Capitol
- Fulham - Oyster Rooms
- Hammersmith - Plough & Harrow
- High Wycombe - William Robert Loosley
- Hull - William Wilberforce
- Ipswich - Golden Lion
- Islington - Glass Works
- Lichfield - Gatehouse
- Lincoln - Forum
- Maidenhead – Greyhound
- Mansfield - Stag & Pheasant
- Milton Keynes - David Garrick
- Mitcham - White Lion of Mortimer
- Newbury - Diamond Tap
- Newcastle-upon-Tyne - Union Rooms
- Newport - Tom Toya Lewis
- Newquay - Cribbar
- North Finchley - Tally Ho
- Nuneaton - Felix Holt
- Putney - Railway
- Reading - Monks' Retreat
- Rugby - Lawrence Sheriff
- Sittingbourne - Summoner
- Stirling – (Proposed pub and hotel site)
- Sunderland - Lambton Worm
- Swindon - Groves Company Inn
- Tamworth - Silk Kite
- Torquay - London Inn
- Trowbridge - Sir Isaac Pitman
- Walsall - Imperial
- Walton on Thames - Regent
- West Kirby - Dee Hotel
- Worthing - Sir Timothy Shelley
Full details of the properties are available at www.jdwdisposals.com.