Carlsberg is "very disappointed" and "surprised" by Enterprise's decision to de-list the lager from its 7,763 pubs from 31 January.
Britain's second biggest pubco has told tenants that Carlsberg is no longer viable because volumes and demand have dropped. It said the market had changed with customers wanting "premium standard beer" such as Beck's Vier and Amstel. It will also offer Stella Artois 4% for the first time.
"At a time when tenants are facing unprecedented challenges and need support, Enterprise has taken a decision to restrict their choice and offer them brands that are not comparable, in that they are significantly less popular with the consumer, are more expensive and have a much lower rate of sale," a Carlsberg spokesman said.
"In addition to this, at a time when value is critical to both licensees and drinkers the alternative products put forward are more expensive."
It added: "Whilst ultimately it is Enterprise's choice to decide which brands they stock, we are not clear why Enterprise Inns has decided that Draught Carlsberg in 'no longer viable' in their business, as they have almost 1,500 tenants choosing to stock the product, which will be more than the majority of draught brands that they list.
"Ultimately, Carlsberg UK's concern is with the Enterprise tenants who have supported our brand for years and who undoubtedly will suffer the most.
"In a very challenging market place with declining beer sales and pubs closing at a rate of around five a day, they will now risk losing valuable customers and will be at a commercial disadvantage to other local pubs that are nearby."